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Comparison of the relative efficiency of atPARP2-catalyzed auto- and DNA ADP-ribosylation; 1 μM atPARP2 and 50 nM human PARP3 were incubated with 10 μM cold oligonucleotide duplexes in the presence of 1 μM [adenylate-32P]-NAD+ for 30 min at 37°C. The reaction products were analyzed by denaturing PAGE. Arrows indicate PAR–DNA, mono-ADPr-p21 mer products and free 21-mer oligonucleotide. For more details, see section “Materials and Methods.”
