Table 1.

Details of Primer sequences.

Gene	Primers	Annealing Temperature	Size of the PCR product
BRAF	FP: 5′GCTTGCTCTGATAGGAAAATGAG-3′	56 °C	237 bp
	RP: 5′GATACTCAGCAGCATCTCAGG-3′
KRAS	FP: 5′-GGCCTGCTGAAAATGACTGA-3′	52 °C	81 bp
	RP: 5′-TAGCTGTATCGTCAAGGCAC-3′
HRAS	FP: 5′-TGA GGA GCG ATG ACG GAA-3′	52 °C	133 bp
	RP: 5′-GCG CTAGGC TCA CCT CTA T-3′
NRAS	FP: 5′-CCT GTT TGTTGG ACA TAC TG-3	52 °C	143 bp
	RP: 5′-CCT GTA GAG GTT AAT ATCCG-3′