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Z-stacking of single plane images improves quantification of dendritic spines. A, A representative single plane image for a segment of a dendritic branch. B, A representative z-stack image summed from 15 different focal fields of all spines on the same dendritic branch (inset 1–15). The z-stack image clearly shows improved visibility and increased quantifiable dendritic length. Scale bar: 10 μm.
