Table 1.
Action potential and passive membrane properties
| HFHS mice | Chow mice | p Value (t score) | WG mice | NWG mice | p Value (t score) | |
|---|---|---|---|---|---|---|
| AP amplitude (mV) | 52.06 ± 12.21 (n = 34) | 46.14 ± 13.79 (n = 25) | p = 0.0868 (t(57) = 1.74) | 48.52 ± 7.46 (n = 10) | 55.82 ± 14.92 (n = 15) | p = 0.1526 (t(23) = 1.48) |
| AP threshold (mV) | −42.7 ± 9.7 (n = 33) | −40.0 ± 2.8 (n = 25) | p = 0.1719 (t(56) = 1.38) | −45.3 ± 5.2 (n = 9) | −43.2 ± 6.7 (n = 15) | p = 0.4254 (t(22) = 0.81) |
| AP half-width (ms) | 1.706 ± 0.829 (n = 34) | 1.258 ± 0.581 (n = 26) | *p = 0.0226 (t(58) = 2.34) | 1.739 ± 0.676 (n = 10) | 1.359 ± 0.484 (n = 15) | p = 0.1140 (t(23) = 1.78) |
| AP rise time (ms) | 1.784 ± 0.915 (n = 34) | 1.181 ± 0.591 (n = 26) | *p = 0.005 (t(58) = 2.92) | 2.103 ± 1.083 (n = 10) | 1.308 ± 0.516 (n = 15) | *p = 0.0213 (t(23) = 2.47) |
| AP decay (ms) | 1.714 ± 0.861 (n = 34) | 1.324 ± 0.747 (n = 26) | p = 0.071 (t(58) = 1.84) | 1.620 ± 0.7162 (n = 10) | 1.443 ± 0.583 (n = 15) | p = 0.5036 (t(23) = 0.68) |
| AHP peak (mV) | −12.9 ± 4.112 (n = 29) | −14.2 ± 3.537 (n = 23) | p = 0.2367 (t(50) = 1.20) | −13.65 ± 2.906 (n = 8) | −12.25 ± 5.08 (n = 14) | p = 0.4867 (t(20) = 0.71) |
| AHP half-width (ms) | 8.281 ± 4.393 (n = 17) | 7.46 ± 2.648 (n = 9) | p = 0.6141 (t(24) = 0.52) | 10.29 ± 3.307 (n = 5) | 7.878 ± 5.006 (n = 8) | p = 0.3631 (t(11) = 0.95) |
| Series resistance (MΩ) | 6.457 ± 2.302 (n = 39) | 6.735 ± 3.257 (n = 26) | p = 0.7114 (t(63) = 0.36) | 6.099 ± 1.968 (n = 9) | 6.583 ± 2.999 (n = 12) | p = 0.521 (t(19) = 0.65) |
| Capacitance (pF) | 7.475 ± 2.009 (n = 26) | 7.816 ± 2.392 (n = 12) | p = 0.6502 (t(36) = 0.46) | 8.077 ± 1.654 (n = 10) | 7.794 ± 1.758 (n = 8) | p = 0.7301 (t(16) = 0.65) |
| Membrane resistance (MΩ) | 366.8 ± 170.9 (n = 26) | 261.6 ± 58.39 (n = 12) | *p = 0.0463 (t(36) = 2.06) | 282.8 ± 154 (n = 10) | 347 ± 138.5 (n = 8) | p = 0.3722 (t(16) = 0.92) |
| Current injected (pA) | −5.24 ± 12.9 (n = 30) | −14.9 ± 8.6 (n = 24) | *p = 0.003 (t(52) = 3.15) | −0.95 ± 10.42 (n = 7) | −7.2 ± 16.2 (n = 14) | p = 0.3682 (t(19) = 0.92) |
All comparisons used two-tailed unpaired t tests. AP, Action potential; AHP, afterhyperpolarization; Current injected, amplitude of current injected in current-clamp mode to maintain membrane potential at approximately −50 mV. The asterisk highlights the tests where p < 0.05.