Vegf-A expression increases in the end target muscle following nerve injury in WT mice. A, Immunostaining of Vegf-A protein (pseudocolored in gray, arrows) was nearly undetectable in naive, uninjured muscles. B–D, From the 3rd to the 12th day postinjury, Vegf-A expression steadily increased. E, The increasing Vegf-A protein expression/image area under 20× magnification was quantitatively summarized for all time points. Vegf-A Ab (gray). Scale bar: 50 μm. Data: mean ± SD; N= 3 mice/time point; *p < 0.05.