Figure 8.

VegfR2/Vegf-A inhibition reduces NMJ reinnervation after nerve injury. A, B, NMJ reinnervation, defined as overlap of axonal staining (NF200 Ab, green) with α‐bungarotoxin (BTX, endplates, red) staining (arrows), was significantly less in CBZ-treated mice compared with controls at day 14, but not at days 21 (C, D) or 28 (E, F) after nerve injury and repair. The insets highlight innervated endplate (A) and denervated endplate (B) in saline-treated and CBZ-treated mice, respectively. G, Quantification analyses showed both full and partial NMJ reinnervation in CBZ-treated mice were approximately half the values of controls at 14 d postinjury. The percentage of fully reinnervated NMJs did not change from 21 to 28 d postinjury in the CBZ group. H, There were no differences in CMAP amplitudes between CBZ-treated mice and saline-treated controls at all time points postinjury. DAPI = nuclear staining (blue). Scale bar: 50 μm. Data: mean ± SD; N = 3–5 mice/treatment/time point.