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. 2020 Dec 9;10:21523. doi: 10.1038/s41598-020-78632-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Plaque composition in terms of CD45 signal. (A) Immune cell marker CD45 signal in ApoE−/− mice in different vessel compartments. CD45 volume is increased in the aortic arch of ApoE−/− mice on western diet (FDR corrected p value = 0.014), but not in the BCA branch (FDR corrected p value = 0.95). (B) Composition of individual plaque objects in terms of CD45 signal and total volume. (C) Optical cross section images reveal that CD45 is only found in the periphery of larger plaques. Left image: aortic arch, right image: BCA. Both images are from Apoe−/− mice fed western diet.