(A) Western blot analysis of P-P65 in MTECs. (B) ELISA of TNF-α and IL-1β in MTECs. Results represent means ± SEM for three independent experiments. (C) Western blot of Col-I, α-SMA, FN and E-cadherin in MTECs. Results represent means ± SEM for three independent experiments. *P<0.05, **P<0.01, ***P<0.001 vs NC. #P<0.05, ##P<0.01, ###P<0.001 vs HG. Abbreviation: MG, 5.5 mM glucose plus mannitol 24.5 mM mannitol. HG, high glucose. CAR, carnosine.