(A) Real-time PCR of GNMT in MTECs. (B) Western blot of GNMT in MTECs. (C) Western blot of KIM-1 and cleaved caspase-3 in MTECs. (D) Western blot of p65 NF-κB phosphorylation, Col-I and α-SMA in MTECs. Results represent means ± SEM for three independent experiments. *P<0.05, ***P<0.001 vs NC. #P<0.05, ##P<0.01, ###P<0.001 vs GNMT-KD group. $$$P<0.001 vs HG. Abbreviations: HG, high glucose; CAR, carnosine; EV, empty vector; KD, knockdown.