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. 2020 Dec 8;24:100846. doi: 10.1016/j.bbrep.2020.100846

Fig. 3.

Fig. 3

Deubiquitinating enzyme regulates RNA binding family proteins: A) Western blotting of SF2/ASF1, hnRNPA1, USP5, α-Tubulin (loading control) was performed in U87 glioma cells transfected with si RNA against USP5 and USP8, were compared with control si RNA. B) T98G glioma cells transfected with two different hnRNPA1 siRNAs compared with control siRNA. Western blotting was performed to analyse the expression of MGMT and SF2/ASF1. C) Telomerase concentration (ng/ml) was measured in U87 glioma cells transfected with specific siRNAs (USP5, USP8, and hnRNPA1) were compared with control si RNA. Statistical analysis was performed. No significant p value ​> ​0.05, if p value ​< ​0.05 (∗), <0.01(∗∗), and <0.001(∗∗∗) are considered significant. D) Comparative mRNA alternative spliced gene variant of BIN1, & RON in USP5 (10 ​nM) and USP8 (10 ​nM) knock down U87 ​cells was determined, GAPDH housekeeping gene was used for loading control. Densitometry was analysed using Image j available (NIH) software. (n ​= ​3, Mean ​± ​SE).