Fig. 7.

Knock down SF2/ASF1, USP5, and co-knockdown SF2/ASF1+USP5 with specific siRNAs were performed and were compared with control siRNA in A172 & T98G glioma cells. Cells were also treated with Bortezomib (1 ​nM) (Proteosome inhibitor) and analysed the NAD and NADP concentration in nM, using colorimetric kit. Absorbance was taken at 450 ​nm.

A) NAD/NADH estimation

B) NADP/NADPH assay

Statistical analysis was performed. No significant p value ​> ​0.05, if p value ​< ​0.05 (∗), <0.01(∗∗), and <0.001(∗∗∗) are considered significant.