Figure EV3. Effects of UK5099 treatment on cellular metabolites.

• A
  Effect of MPC inhibition on polar metabolite abundance. Primary brown adipocytes were treated for 24 h with vehicle (DMSO) or 10 µM UK5099. Data show total metabolite abundance measured by GC‐MS from n = 6 individual experiments. Data were normalized to vehicle for each individual experiment. *P < 0.05, **P < 0.01 compared to vehicle by Student’s t‐test.
• B, C
  Effect of MPC inhibition on glutamine catabolism and contribution to TCA cycle metabolites. (B) Schematic representation of metabolite tracing using [U‐¹³C₅] glutamine. (C) [U‐¹³C₅] glutamine tracing in primary brown adipocytes treated with vehicle (DMSO), or 10 µM UK5099. Data show mole percent enrichment (MPE) of isotope‐labeled substrate into respective metabolite from n = 4 individual experiments. *P < 0.05, **P < 0.01 compared to vehicle by Student’s t‐test.
• D
  Quantification of percent M + 3 label from [U‐¹³C₅] glutamine into respective metabolite from experiment described in (C) (n = 4 individual experiments). *P < 0.05, **P < 0.01, ***P < 0.001 compared to vehicle by Student’s t‐test.

Data information: All data are presented as mean ± SEM.