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. 2020 Oct 19;21(12):e49684. doi: 10.15252/embr.201949684

Figure 3. The anticodon stem‐loop is required for mascRNA‐QARS interaction.

Figure 3

  1. Biotin‐labeled mascRNA (sense [S] and antisense [AS]) and the acceptor stem mutants (Mut1–8) were used to pulldown the RNA–protein complexes. Top panel: Western blotting of QARS in the pulldown samples. β‐Tubulin was used as an cytosolic input control for the pulldown. Bottom panel: biotin detection of the biotin‐labeled mascRNA and the mutants.
  2. Biotin‐labeled mascRNA (sense (S) and antisense [AS]) and the anticodon stem‐loop mutants (Anti‐Mut1–9) were used to pulldown the RNA–protein complexes. Top panel: Western blotting of QARS in the pulldown samples. β‐Tubulin was used as an cytosolic input control for the pulldown. Bottom panel: biotin detection of the biotin‐labeled mascRNA and the mutants.
  3. The secondary structure of mascRNA with the acceptor stem and the anticodon stem‐loop in red boxes.
  4. The acceptor stems of mascRNA and the mutants with the mutated nucleotides in red.
  5. The anticodon stem‐loops of mascRNA and the mutants with the mutated nucleotides in red.
  6. Gel shift assay of biotin‐labeled mascRNA or the mutants (Anti‐Mut1 and Mut1) with the cytosol.
  7. Comparison of human mascRNA and tRNAGln UUG secondary structures. The identical nucleotides were marked in red.