Skip to main content
. 2020 Oct 19;21(12):e49684. doi: 10.15252/embr.201949684

Figure EV5. mascRNA promotes cell proliferation through QARS .

Figure EV5

  1. qRT–PCR analysis of MALAT1 levels in the lysates of HEK cells before (Control) and after (Starvation) FBS starvation.
  2. qRT–PCR analysis of MALAT1 levels in the human embryonic stem cells on different days of differentiation (D2, D3, D5, D6, D7, D8, D9).
  3. Soft agar colony formation assay of HEK cells overexpressing mascRNA, or expressing the mutants (Anti‐Mut1 and Mut1) or the scrambled RNA (Scramble).
  4. Soft agar colony formation assay of QARS (shQARS) or GARS (shGARS) knockdown cells and the control cells stably overexpressing mascRNA (mascRNA) or expressing the scrambled RNA (Scramble).
  5. Soft agar colony formation assay of MALAT1 knockdown cells (shMALAT1) and the control cells (Control) with stable QARS (QARS) or GARS (GARS) overexpression or without (PQ).
Data information: Statistical comparisons are performed using unpaired t‐tests (biological replicates n = 3); **P < 0.01; ***P < 0.001. Data are presented as mean ± standard error of the mean (s.e.m.).