Figure 2.
Effects of solvent fractions on ABTS radical scavenging, anti-tyrosinase, and anti-melanogenesis activities. (A) ABTS radical scavenging activity was determined by measuring the absorbance of mixtures of the ABTS radical and the indicated concentrations of solvent fractions. (B) Tyrosinase activity was determined by the l-DOPA oxidation assay in the presence of solvent fractions at the indicated concentrations. (C) Cell viability was determined by the MTT assay in the presence of 0.2 mg/mL solvent fractions. (D) Melanin content in B16F10 cells was determined using a photometric method, as described in the Materials and Methods section. (E) Cellular tyrosinase activity was determined by measuring dopachrome resulting from the enzymatic reaction of cell lysates with l-DOPA. (F) Scavenging activity of L. thunbergianus solvent extracts and trolox on hydrogen peroxide-mediated intracellular ROS was determined using H2DCFDA. Data are expressed as the mean ± SD of three independent experiments.