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. 2020 Oct 7;21(12):e50733. doi: 10.15252/embr.202050733

Figure 5. Atg9 and the Dsl1 complex independently contribute to the autophagosome biogenesis.

Figure 5

  • A, B
    Quantification of size and number of autophagosomes in the indicated ts mutant strains. Error bars represent standard deviation of 3 independent experiments. GFP‐tagged Atg8 in the different mutants were grown at 23°C (ts mutant) or 30°C (atg1∆) in SDC and then shifted to SD‐N for 2 h at 30°C (atg1∆) or 37°C (ts mutant), and analyzed by fluorescence microscopy. Scale bar, 1 μm.
  • C
    Localization of Atg8 relative to Atg9 during nitrogen starvation. dsl3 cells expressing mCherry‐tagged Atg9 and carrying a CEN plasmid expressing GFP‐Atg8 were grown at 23°C in SDC and then shifted to SD‐N for 2 h at 24°C or 37°C. Cells were analyzed by fluorescence microscopy. Scale bar, 5 μm. Scale bar for inset is 0.6 μm.
  • C
    Atg9 localization in dsl3 cells during starvation. dsl3 ts cells expressing mCherry‐tagged Atg9 and carrying a CEN plasmid expressing GFP‐Atg8 and plasmid pRS315‐CUP1pr‐BFP-APE1 were grown in SDC medium containing CuSO4 to induce formation of the giant Ape1 structure and starved for 1 h. Cells were analyzed by fluorescence microscopy. Scale bar, 5 μm. Scale bar for inset is 0.6 μm.