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. 2020 Oct 14;21(12):e50642. doi: 10.15252/embr.202050642

Figure EV5. Depletion and overexpression of Merlin in various cells.

Figure EV5

  1. Met5‐A cells stably transduced with the indicated shRNAs against Merlin or a scrambled shRNA were subjected to Western blotting.
  2. Met5‐A cells stably transduced with the indicated shRNAs against NEDD4L or a scrambled shRNA were subjected to Western blotting.
  3. Meso‐33 cells transduced with vector or the indicated Merlin forms were subjected to Western blotting. The ratio of mono‐ubiquitinated to native Merlin in each lane was quantified by ImageJ and is shown under the blot.
  4. Meso‐33 cells stably transduced with the indicated shRNAs targeting NEDD4L or a scrambled shRNA were detached by using the procedure described in the Materials and Methods and subjected to Western blotting.
  5. Meso‐33 cells stably transduced with the indicated shRNAs against NEDD4L or a scrambled shRNA were then transduced with vector or Merlin prior to Western blotting.
  6. FC‐1801 cells stably transduced with vector or the indicated Merlin forms were subjected to Western blotting.

Source data are available online for this figure.