Figure EV3. Induction of NF‐κB‐dependent genes upon H5N1 infection.

• A–X
  THP‐1 cells were mock treated or infected with the VN Wt and ΔF2 strains for 4 h (A–H), 8 h (I–O), 18 h (P), and 24 h (Q–X) at MOI MOI 6 (I–O)‐10 (A–H and P–X). Relative expression levels normalized to the average of mock were determined by RT–qPCR for indicated mRNAs normalized to 18S rRNA and calibrated to mock. The mean ± standard deviation of 2–6 independent experiments with at least two technical replicates each is shown (each dot represents an independent biological sample). Statistical analysis was performed by paired two‐tailed Student’s t‐test, and P‐values are indicated.