Figure EV4. IAV dependent activation of the NLRP3 inflammasome and caspase 3 after 12 and 24 h.

1. Supernatants of THP‐1 cells infected with VN Wt and ΔF2 virus at a MOI 10 for 12 h or treated with 6.7 μM of nigericin for 45 min or treated with 1 µM of staurosporine for 12 h or 24 h were analyzed by immunoblotting against gasdermin D, caspase 1, NP, and beta actin (n = 3).
2. Supernatants and cell lysates of THP‐1 cells infected with VN Wt and ΔF2 virus at a MOI 10 for 12 h or treated with 6.7 μM of nigericin for 45 min or treated with 1 µM of staurosporin for 12 h were analyzed by immunoblotting against caspase 1, NP, and beta actin (n = 3).

Source data are available online for this figure.