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. 2020 Dec 9;20:593. doi: 10.1186/s12935-020-01694-x

Fig. 2.

Fig. 2

CircPLK1 knockdown limited BC cell growth, migration and invasion. BT549 and HCC38 cells were transfected with sh-NC or sh-circPLK1. a Knockdown efficiency of circPLK1 was evaluated by qRT-PCR. b CCK-8 assay was employed to evaluate cell viability. c, d Flow cytometry was applied to analyze the cell cycle distribution. e Western blot assay was conducted to examine the protein expression of CDK4 and CDK6. f, g Transwell assay was utilized to count the number of migrated and invaded cells (100 ×). ***P < 0.001