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. 2020 Dec 9;20:593. doi: 10.1186/s12935-020-01694-x

Fig. 4.

Fig. 4

CircPLK1 silence inhibited BC cell growth, migration and invasion by downregulating IGF1. BT549 and HCC38 cells were transfected with sh-NC, sh-circPLK1, sh-circPLK1 + pcDNA3.0-NC, or sh-circPLK1 + pcDNA3.0-IGF1. a CCK-8 assay was employed to evaluate cell viability. b Cell cycle distribution was determined by flow cytometry. c, d The expression levels of CDK4 and CDK6 were analyzed by western blot analysis. e, f Cell migration and invasion were evaluated by transwell assay. ***P < 0.001