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. 2020 Dec 9;20:593. doi: 10.1186/s12935-020-01694-x

Fig. 6.

Fig. 6

MiR-4500 exerted its anti-cancer effects in BC cells by targeting IGF1. BT549 and HCC38 cells were transfected with miR-NC, miR-4500, miR-4500 + pcDNA3.0-NC, or miR-4500 + pcDNA3.0-IGF1. a Cell viability was assessed by CCK-8 assay. b Flow cytometry was applied to analyze the cell cycle distribution. c, d Western blot assay was performed to measure the protein levels of CDK4 and CDK6. e, f Transwell assay was employed to determine cell migration and invasion. ***P < 0.001