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. 2020 Sep 2;9(18):e017533. doi: 10.1161/JAHA.120.017533

Figure 1. Generation of global (Rgs5 −/−) and endothelial cell–specific regulator of G‐protein signaling 5 (RGS5) deficient mice (EC‐Rgs5 –/–).

Figure 1

A, Western blotting of RGS5 in the brain, lung, heart, and liver of Rgs5 −/− mice compared with control, transgenic mice expressing Cre recombinase under the control of the phosphoglycerate kinase 1 promoter (Pgk‐Cre). B, Blood pressure and heart rate of Rgs5 −/− and Pgk‐Cre mice (n=5). Western blot analysis of RGS5 expression in primary brain endothelial cells (C) and brain, heart, and lung of EC‐Rgs5 –/– mice (D), with and without (Control) tamoxifen treatment (n=5). E, Blood pressure (BP) and heart rate of Control and EC‐Rgs5 –/– mice (n=5–4). The Welch t test was used for normally distributed data, and the Mann‐Whitney U test was used for all nonnormally distributed data set comparisons. *P<0.05, **P<0.01. B.p.m. indicates beats per minute.