Table 1.
Performance of the different rapid identification methods using MALDI-TOF–MS on positive blood cultures
| References | Methods | Sensibility (%) | Estimated technical time | ||
|---|---|---|---|---|---|
| Commercial kits | |||||
| [44] | Sepsityper kit® (Bruker Daltonics) | 75.6% (n = 160) | 35 min (5 centrifugation steps) | ||
| [45] | 80.8% (n = 411) | ||||
| [30] | 79.8% (n = 3320) | ||||
| [46] | Vitek MS Blood culture kit® (Biomérieux) | 73% (n = 259) | 15 min (No centrifugation steps) | ||
| [47] | Rapid BACpro®II kit (Nittobo Medical Co) | 76.5% (n = 17) | 15 min (4 centrifugation steps) | ||
| [43] | Rapid BACpro®II kit (Nittobo Medical Co) improvement | 96,3% (n = 269) | |||
| This study |
Rapid Sepsityper® protocol (Bruker Daltonics): RS ± FA Complete Sepsityper® protocol on unidentified samples (n = 94) |
68.6% (n = 299) 78.6% (n = 299) |
10 min (2 centrifugation steps) 35 min (5 centrifugation steps) |
||
| In-house protocols: centrifugation | |||||
| [48] | Centrifugation | 95% (n = 277) | > 20 min (> 5 centrifugation steps) | ||
| [41] | Centrifugation | 43% (n = 79) | > 10 min (4 centrifugation steps) | ||
| [49] | Centrifugation | 85.9% (n = 85) | 15 min (2 centrifugation steps) | ||
| In-house protocols: centrifugation in Separator tube | |||||
| [50] | Clot activator and gel BD Vacutainer tubes® (BD Diagnostics) ACUETTE® Z Serum Sept Clot Activator (Greiner Bio-One) | 89.6% (n = 532) | > 20 min (5 centrifugation steps) | ||
| [51] | ACUETTE® Z Serum Sept Clot Activator (Greiner Bio-One) | 90% (n = 186) | 15 min (2 centrifugation steps) | ||
| [42] | Separator tube with plasma separation gel | 88.7% (n = 789) | > 20 min (2 centrifugation steps) | ||
| [52] | Serum Separator tube (BD Diagnostics) | 68.7% (n = 195) | > 30 min (5 centrifugation steps) | ||
| In-house protocols: centrifugation + lysis reagent | |||||
| [53] |
5% Saponin lysis (fast protocol) 20% SDS lysis (fast protocol) |
53% (n = 42) 86% (n = 42) |
20 min (2 centrifugation steps) | ||
| [54] | 0.6% polyoxyethylene 10 oleoyl ether (Brij 97) in 0.4 mol/L 3-cyclohexylamino-1-propane sulfonic acid lysis | 82,4% (n = 125) | > 10 min (2 centrifugation steps) | ||
| [34] | Triton X-100 lysis | 80.5% (n = 681) | 10 min (2 centrifugation steps) | ||
| In-house protocols: Centrifugation + lysis reagent + additional protein extraction | |||||
| [55] |
Saponin lysis + 10% TFA extraction |
61.4% (n = 127) | 20 min (2 centrifugation steps) | ||
| [56] |
5% Saponin lysis + Ethanol/FA extraction |
80.6% (n = 155) | 20 min (2 centrifugation steps) | ||
| [57] |
5% Saponin lysis 10% SDS lysis + 100% Ethanol/100% Acetonitrile/70% FA extraction |
79.5% (n = 176) 88% (n = 176) | > 20 min (5 centrifugation steps) | ||
| [41] |
10% SDS lysis + 70% FA/Acetonitrile extraction |
49% (n = 101) | > 20 min (4 centrifugation steps) | ||
| [58] |
Ammonium chloride lysis + 70% Ethanol/70% FA extraction |
78.7% (n = 122) | 30–45 min (4 centrifugation steps) | ||
| [59] |
0.1% TFA lysis + 70% FA/100% Acetonitrile extraction |
88.9% (n = 245) | > 40 min (6 centrifugation steps) | ||
| [40] |
0.2% Triton X-100 + 0.1% SDS lysis 0.2% Triton X-100 + 1.8% SDS lysis + Ethanol/100% Acetonitrile/70% FA extraction + 70% FA extraction |
86.3% (n = 450) 86.4% (n = 59) |
30 min (5 centrifugation steps) 10 min (3 centrifugation steps) |
||
The technical time is either directly issued from publications when available or estimated from the number of centrifugation and centrifugation time described in protocols (estimated as > to x min with centrifugation steps between 1 to 3 min). (SDS: Sodium dodecyl sulfate; FA, formic acid; TFA, trifluoroacetic acid; n, number of samples tested)