Table 1.
Antibiotic | Solvent | Stock concentration (mg/mL) |
Final concentration (μg/mL) |
---|---|---|---|
Spectinomycin | Deionized water | 50 | 50 |
Chloramphenicol | 100% Ethanol | 25 | 25 |
Kanamycin | Deionized water | 100 | 250 |
Gentamicin | Deionized water | 50 | 100 |
All stock solutions should be sterilized by passing through 0.22 μM filters and stored at −20°C as aliquots.