Figure 1. Time Course of SARS-CoV-2 Antibody Binding and Neutralization Responses after mRNA-1273 Vaccination.

Shown are data from 34 participants who were stratified according to age: 18 to 55 years of age (15 participants), 56 to 70 years of age (9 participants), and 71 years of age or older (10 participants). All the participants received 100 μg of mRNA-1273 on days 1 and 29, indicated by arrows. The titers shown are the binding to spike receptor–binding domain (RBD) protein (the end-point dilution titer) assessed on enzyme-linked immunosorbent assay (ELISA) on days 1, 15, 29, 36, 43, 57, and 119 (Panel A); the 50% inhibitory dilution (ID₅₀) titer on pseudovirus neutralization assay on days 1, 15, 29, 36, 43, 57, and 119 (Panel B); the ID₅₀ titer on focus reduction neutralization test mNeonGreen (FRNT-mNG) assay on days 1, 29, 43, and 119 (Panel C); and the 80% inhibitory dilution (ID₈₀) titer on plaque-reduction neutralization testing (PRNT) assay on days 1, 43, and 119 (Panel D). Data for days 43 and 57 are missing for 1 participant in the 18-to-55-year stratum for whom samples were not obtained at those time points. Each line represents a single participant over time.