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. 2020 Dec 4;2020(1):67–75. doi: 10.1182/hematology.2020000090

Table 2.

Comparison of available assays on the TEG 5000 and ROTEM Delta analyzers

Assays Interpretation/clinical indications
TEG 5000* ROTEM Delta†

  • Standard TEG (kaolin)

  • Reagent contains kaolin activator.

  • INTEM

  • Reagent contains ellagic acid activator.

 These assays assess the contact activation pathway of coagulation and provide information similar to the activated partial thromboplastin time (APTT).
N/A

  • EXTEM

  • Reagent contains tissue factor activator.

 This assay assesses the tissue factor–initiated pathway of coagulation and provides information similar to the prothrombin time (PT).

  • Rapid TEG (rTEG)

  • Reagent contains both tissue factor and kaolin as activators.

 N/A This assay assesses both the tissue factor–initiated and contact pathway–initiated coagulation and provides information similar to the PT and APTT.

  • Heparinase TEG (hTEG)

  • Reagent contains kaolin activator and lyophilized heparinase for neutralizing unfractionated heparin.

  • HEPTEM

  • Reagent contains ellagic acid activator and lyophilized heparinase for neutralizing unfractionated heparin.

 These assays can assess heparin’s effect when used in conjunction with the kaolin reagent (Standard TEG) and compared with the kaolin analysis on the TEG analyzer or when used in conjunction with the INTEM assay and compared with the INTEM analysis on the ROTEM analyzer.

  • Functional fibrinogen TEG (FLEV-TEG)

  • Reagent contains tissue factor and abciximab, a GPIIb/IIIa inhibitor, to block the platelet contribution to clot formation.

  • FIBTEM

  • Reagent contains tissue factor and cytochalasin D, an actin polymerization inhibitor, to block the platelet contribution to clot formation.

 These assays can qualitatively assess the contribution of fibrinogen to clot strength independent of platelets when used in conjunction with the kaolin reagent (Standard TEG) and compared with the kaolin analysis on the TEG analyzer or when used in conjunction with the EXTEM assay and compared with the EXTEM analysis on the ROTEM analyzer.
N/A

  • APTEM

  • Reagent contains aprotinin (fibrinolysis inhibitor).

 This assay is designed to allow discrimination between fibrinolysis and platelet-mediated clot retraction.

  • TEG platelet mapping (TEGPM)

  • Reagent contains ADP or arachidonic acid.

 N/A This assay is designed to assess platelet function and the effect of antiplatelet agents.

  • Native TEG

  • Native whole blood sample analyzed after recalcification

  • NATEM

  • Native whole blood sample analyzed after recalcification

 These assays are impractical for clinical use, given their long reaction rate (R) on the TEG analyzer and clot formation time (CFT) on the ROTEM analyzer, respectively. They can be used to run custom hemostasis tests.

GP, glycoprotein; N/A, corresponding assay is not available on analyzer; ROTEM, rotational thromboelastometry; TEG, thromboelastography.

*

TEG 6S analyzer: Standard TEG (kaolin) is CK channel; rapid TEG (rTEG) is CRT channel; heparinase TEG (hTEG) is CKH channel; and functional fibrinogen TEG (FLEV-TEG) is CFF channel.

†

ROTEM Sigma analyzer: Assays have the same designation as the ROTEM Delta analyzer, except with a suffix C at the end (eg, EXTEM C).