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Preparation of orthodontic aligners used in the study. Conventional orthodontic plaster models were ground plane in the region of buccal areas. Required diameter of 6 mm of flat surface was confirmed by a conventional orthodontic caliper (A). Flat aligner surface of 6 mm equivalent to buccal plaster cast surface (B,C). Discs of 6 mm in diameter were cut out of regions with flat crown surfaces and used in cell culture experiments (D).
