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. 2020 Nov 24;13(23):5311. doi: 10.3390/ma13235311

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Gene expression of inflammatory markers. Gene expression of IL-6 (A), IL-8 (B) and ICAM1 (C) after two and seven days in Ca9-22 cells grown on different aligners’ surfaces were measured by qPCR. Y-axes represent n-fold expression in relation to Ca9-22 cells grown on TCP (n-fold expression = 1), calculated using 2^−ΔΔCt method. Data are presented as the mean ± s.e.m. of four independent experiments. †—significantly higher compared to control; *—significantly lower compared to control; #—significantly different between groups. p < 0.05.