Table 4.

The most commonly used additives that promote differentiation of iPSCs into neural precursor cells (NPCs) and, further, to motor neurons (MNs).

NPC Differentiation	Motor Neuron Maturation
Neural induction medium 10% KnockOut serum NEAA LAA SB431542 CHIR99021 Dorsomorphin bFGF EGF B27 1% penicillin/streptomycin	Neural induction medium B27 N-2 NEAA LAA RA SHH SAG Purmorhamine CNTF BDNF NT-3 GDNF

SAG—Smoothened Agonist, CNTF—Ciliary Neurotrophic Factor, NT-3—Neurotrophin-3, GDNF—Glial Cell-Derived Neurotrophic Factor.