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. 2020 Nov 24;21(23):8916. doi: 10.3390/ijms21238916

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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α-synuclein fibril and ThT solution optical density at 600 nm (A), bound ThT concentration (B), intensity/bound ThT (I/c_B) ratio (C) and fluorescence intensity (D) at different NaCl concentrations. Optical density and fluorescence spectra intensities are the result of three repeats. The white area in the bound ThT graph (B) represents conditions under which bound ThT concentration could not be determined accurately. Fluorescence intensity values are corrected for the primary and secondary inner filter effects.