Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Nov 25;21(23):8951. doi: 10.3390/ijms21238951

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Examples of immunofluorescent staining for FerrH, NeuN (mature neurons), DCX (young neurons), and activated microglia\macrophages (CD68). (a) Double-immunofluorescence images stained for mature (FerrH\NeuN for the Ferr-MCAO and PBS-MCAO group, eGFP\NeuN for the eGFP-MCAO group), young (FerrH\DCX for the FerrH-MCAO and PBS-MCAO group, and eGFP\DCX for the eGFP-MCAO group) neurons in the zone near the lateral ventricle and SVZ. (b) Double-immunofluorescence images stained for microglia\macrophages (FerrH\CD68) in the near lateral ventricle and SVZ (left three rows) and inside the ischemic lesion (right three rows). For eGFP-MCAO group, FerrH is visualized by far-red fluorochrome and is shown as green pseudo color. Scale bars corresponds to 50 µm. Abbreviations: MCAO, middle-cerebral-artery occlusion; AAV, adeno-associated viral backbone; pDCX, doublecortin promoter; FerrH, ferritin heavy chain; eGFP, enhanced green fluorescent protein; PBS, phosphate-buffered saline; SVZ, subventricular zone.