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The processing steps in analysis of MRI and fluorescent images: (1) creating a binary image based on a fluorescent micrograph, (2) reducing the dimension of a binary image to the dimension of T2* image, (3) separation of brain zones based on the signal hyperintensity on T2-weighted images following by T2* signal intensity measurement, (4) scale-up regions of interest (ROIs) to measure IF and calculate labeled cells in micrographs (5).
