Figure 2.

Suppressive effects of rice bran and vitamin B6 on HIF-1α stabilization. Representative immunoblot images and quantitative analyses (n = 4 per group) for HIF-1α and β-Actin showed that HIF-1α was stabilized in ARPE-19 cells under a CoCl₂-induced pseudo-hypoxic condition. Rice bran (1 mg/mL) and vitamin B6 (1 mg/mL) significantly decreased stabilized HIF-1α expression. *** p < 0.001, compared with no treatment, ## p < 0.01, ### p < 0.001, compared with CoCl₂ treatment. Bar graphs were presented as mean ± standard deviation. The data were analyzed using one-way ANOVA followed by a Bonferroni post hoc test. Solvents, rice bran: DMSO; vitamin B6: water.