Figure 4.

Suppression of neovascularization by rice bran and vitamin B6. (A) A schematic illustration demonstrates the murine choroidal neovascularization (CNV) model procedure and administration of rice bran or vitamin B6 to mice. (B) An immunoblot image for HIF-1α and β-Actin in the retina with or without the supplement of rice bran or vitamin B6, 3 days after the laser irradiation. (C) Representative images of CNV in the whole mount staining with isolectin B4 and quantitative analyses (n = 5–6 per group, n = 42–49 laser spots in the eyes per group) showed that the volume of CNV was significantly reduced by administration of rice bran (587.5 mg/kg diet weight) and vitamin B6 (9 or 35 mg/kg diet weight), respectively. Scale bars, 100 μm. * p < 0.05, ** p < 0.01. Dot plot graphs were presented as mean. The data were analyzed using Student’s t-test.