Table 2.

The immune endocannabinoid system.

	CB1 Receptors (Species; Method of Detection)	CB2 Receptors (Species; Method of Detection)	MGL (Species; Method of Detection)	FAAH (Species; Method of Detection)	Production of Endocannabinoids
PBMC	-human; PCR, FC, WB; T cells activated with TNFalpha [149] -human; PCR; [172] -human; PCR; [161]	-human; PCR, FC, WB; T cells activated with TNF-α; [149] -human; PCR; CB2 3 x higher than CB1; [172] -human; PCR; [161]
Lymphocytes	-human; PCR, WB [173]			-human; ELISA, PCR [173]	AEA [173] 2-AG [174]
T cells	-human; PCR; T cells activated with CD3/28; [175] -human; PCR; T cells activated with TNF-α; [149] -human; PCR; [176]	-human; PCR; T cells activated with CD3/28; [175] -human; PCR [16] -human; PCR; T cells activated with TNFalpha [149] -human; FC; [177] -human; PCR; [176]		-human; PCR; [176]
B cells	-human; PCR; [176]	-human; PCR; [16] -human; FC; [177] -human; FC; [178] -human; PCR; [176] -human, FC; [179]		-human; PCR; [176]
Monocytes	-human THP monocytes; PCR; [161]	-human; PCR; [16] -human THP monocytes; PCR; [161] -human, FC; [179]	-human; WB; [180]		2-AG [174]
Macrophages	-human PMA-treated monocyte-derived macrophages; PCR; [161] -mouse RAW264.7 cells; PCR; [161] -human PBMC-derived macrophages; PCR; [181] -rat; circulating macrophages; PCR; [182]	-human; PCR; [16] -human PMA-treated monocyte-derived macrophages; PCR; [161] -mouse RAW264.7 cells; PCR; [161] -human; differentiated monocytes; PCR; [183] -mouse tumor-associated macrophages; PCR; [35]	-mouse; tumor associated macrophages; [35]	-rat; circulating macrophages; PCR; [182]	AEA in RBL-2H3 basophils, J774 and RAW264.7 mouse macrophages [182,184,185,186] 2-AG in mouse peritoneal macrophages [187] 2-AG in J774 cells [182] 2-AG in mouse P388D1 macrophages [188] 2-AG in mouse peritoneal macrophages [189] 2-AG in RAW264.7 cells [186]
NK cells	-human; PCR; [176]	-human; PCR; [16] -human; FC; [177] -human; PCR; [176] -human, FC; [179]		-human; PCR; [176]
Dendritic cells	-human; PCR, WB; [190]	-human; PCR, WB; [190]		-human; PCR, WB; [190]	AEA, 2-AG [190]
Neutrophils	-mouse bone marrow neutrophils (liver injury model); PCR; IF; [191]	-human; PCR; [16] -human; FC; [169] -not detected [168] -mouse bone marrow neutrophils (liver injury model); PCR; IF; [191] -human; WB; [192] -human, FC; [179]			2-AG [174]
Eosinophils		-human; PCR; [168] -human; PCR, Northern Blot; [167] -human; FC, [166]	-human; PCR; [168]		2-AG [174]
Mast cells	-rat RBL2H3 cells; PCR; [193] -mouse (primary BMMCs); WB; [194] -human mucosal-type mast cells; IHC; [171]	-rat RBL2H3 cells; PCR; [193] -mouse (primary BMMCs); WB; [194]		-human mast cells (HMC-1); FAAH activity measured; [195]

AEA, anandamide; 2-AG, 2 arachidonoylglycerol; BMMC, bone marrow-derived mast cell; CB₁, cannabinoid receptor 1; CB₂, cannabinoid receptor 2; ELISA, enzyme-linked immunosorbent assay; FAAH, fatty acid amide. hydrolase; FC, flow cytometry; IF, immunofluorescence; IHC, immunohistochemistry; MGL, monoacylglycerol lipase; PBMC, peripheral blood mononuclear cells; PCR, polymerase chain reaction; PMA, phorbol 12-myristate. 13-acetate; WB, Western blot.