Fig 2. Distribution of the Sweet Home Alabama Cave Crayfish (Cambarus speleocoopi) (blue dots) and eDNA sampling sites (white dots and numbered blue dots) in northern Alabama, USA.

Cambarus speleocoopi eDNA was detected (black triangle) at two historical sites and three new sites. Site numbers correspond to those listed in Table 1. Sites 17 and 18 are historical sites but we could not gain permission to sample. Karst and cave-bearing carbonate strata is shown in gray.