Table 1. Sampling sites where water samples were collected in Jackson and Marshall counties, Alabama, USA and results of screening of an eDNA assay for Cambarus speleocoopi.
| Site no. | County | Site name | Collection date | Technical replicates |
|---|---|---|---|---|
| 1 | Marshall | Ashburn Spring | 11 Jan 2019 | 0/12 |
| 2 | Marshall | Babe Wright Spring | 07 Feb 2019 | 0/24 |
| 3 | Marshall | Beech Spring Cave (spring)a | 29 Apr 2019 | 6/12 |
| 4 | Marshall | Cathedral Caverns | 07 Feb 2019 | 0/12 |
| 5 | Marshall | Cathedral Caverns (spring) | 07 Feb 2019 | 0/12 |
| 6 | Marshall | Cherry Hollow Cavea | 18 Jan 2019 | 5/12 |
| 7 | Marshall | Cherry Hollow Cave (spring)a | 18 Jan 2019 | 3/12 |
| 8 | Marshall | Cushion Spring | 11 Jan 2019 | 2/12 |
| 9 | Marshall | Davis Spring | 11 Jan 2019 | 1/18 |
| 10 | Marshall | Guffey Cave | 22 Feb 2019 | 0/12 |
| 11 | Marshall | Kings Spring Cave | 01 Feb 2019 | 0/18 |
| 12 | Marshall | McGehee Spring | 11 Jan 2019 | 1/12 |
| 13 | Marshall | New Hope Spring | 31 Jan 2019 | 0/12 |
| 14 | Jackson | Bluff River Caveb | 18 Aug 2018 | 0/12 |
| 15 | Jackson | Tumbling Rock Caveb | 04 Mar 2018 | 0/12 |
| 16 | Jackson | Tumbling Rock Cave (spring)b | 04 Mar 2018 | 0/12 |
| 17 | Marshall | Keller’s Cave a | ||
| 18 | Marshall | Porches Spring Cave a |
aHistorical sites for C. speleocoopi.
bSites used for negative field controls. Two to four water samples were collected from a site, each with six PCR technical replicates. Sites 17 and 18 are historical sites but we could not gain permission to sample.