Table 2. Primers and probe developed and used in the current study to amplify a 163-bp fragment of CO1 for Cambarus speleocoopi.

Oligo	Sequence (5’ to 3’)	Direction	Length (bp)	Tm (°C)
Forward	TGGGATAGTTGGGACTTCA	Sense	19	60
Reverse	ATTRCCAAACCCTCCAATTA	Antisense	20	60
Probe	TCCGAGTTGAATTGGGTCAGGTAGGAAGG	Sense	29	70