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. 2020 Nov 30;16(11):e1009028. doi: 10.1371/journal.ppat.1009028

Fig 2. HPV16 evades cGAS/STING responses in HFKs during initial infection.

Fig 2

cGAS/STING responses to pGL3 and wildtype HPV in primary HFKs. (A) HFKs were either transfected for 90 min with 500 ng pGL3 or infected for the duration of the experiment with 1900 ng L1 (equivalent to 500 ng DNA) of HPV virions. Cells were harvested at various times post-treatment and cGAS/STING responses assessed via western blotting. (A) IRF3 was phosphorylated 4 hr post pGL3 transfection, while IRF3 was not phosphorylated at any time post HPV infection with HPV PsV. representative blot of n = 3 biological replicates. (B) Cells were either transfected for 90 min with 250 ng pGL3 or infected for the duration of the experiment with an equivalent amount of HPV virions. Luciferase activity was measured 24 hr post-treatment, normalized to GAPDH, and corrected for pGL3 encapsidation as described in Materials and Methods. *P < 0.05 by unpaired t-test, n = 3 biological replicates.