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. 2020 Nov 26;21(23):8966. doi: 10.3390/ijms21238966

Figure 3.

Figure 3

Time-dependent effect of hyperosmotic stress on autophagy activation and inflammation in primary HCECs. (A) Western blot showed that the protein production of Beclin1, Atg5 and Atg7, as well as LC3B turnover increased while production of p62/SQSTM1 decreased significantly in 24–48 h after HCECs were exposed to 450 mOsM medium, with β-Actin serving as internal control. (B) Quantitative ratio of LC3B II/β-Actin showing increased LC3B turnover in 24–48 h after HCECs were exposed to 450 mOsM. (C–F) ELISA analyses. The pro-inflammatory cytokines, TNF-α (C), IL-1β (D) and IL-6 (E), as well as chemokine IL-8 (F) was suppressed after 24 h in HCECs exposed to 450 mOsM. Data are mean ± SD of three independent experiments. * p < 0.05, compared within groups.