Figure 1.

Leptin downregulated the cathepsin A (CTSA) gene in canine inflammatory mammary adenocarcinoma (CHMp) cells. (a) CTSA gene expression in CHMp cells treated with leptin and Allo-aca was evaluated using real-time PCR. (b,c) Western blot analysis of time-course CTSA/lysosomal-associated membrane protein 2a (LAMP2a) alteration induced by leptin (b) and Allo-aca (c) treatment. (d) Western blot analysis of chaperone-mediated autophagy (CMA)-related genes. Cells were incubated with leptin and/or Allo-aca for 24 h, and CTSA small interfering ribonucleic acid (siRNA) was transfected 24 h before chemical treatment. All graphs are visualized as mean ± standard error of the mean (SEM) with at least three replicates. The column bars with different alphabetical letters indicate significant difference among groups (p < 0.05). Combi: combination of leptin and Allo-aca; NC: negative control siRNA.