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. 2020 Nov 26;9:e56187. doi: 10.7554/eLife.56187

Appendix 1—key resources table.

Reagent type
(species) or resource		 Designation Source or reference Identifiers Additional information
Antibody anti-GFP
(Chicken polyclonal)		 Aves Labs, INC. Cat#GFP-1020,
RRID:AB_2307313 		IF(1:2000)
Antibody anti-V5
(Mouse monoclonal)		 ThermoFisher Scientific Cat#R960-25,
RRID:AB_2556564 		IF(1:500)
Antibody anti-Ase
(Rabbit polyclonal)		 Weng et al., 2010 doi: 10.1016/j.devcel.2009.12.007. IF(1:400)
Antibody anti-Hamlet (Rabbit polyclonal) Eroglu et al., 2014 doi: 10.1016/j.cell.2014.01.053. IF(1:50)
Antibody anti-Dpn
(Rat monoclonal)		 Lee et al., 2006a doi: 10.1038/nature04299. clone 11D1BC7.14 IF(1:2)
Antibody Alexa Fluor 488 AffiniPure Anti-Chicken IgY (IgG) (H+L)
(Donkey polyclonal)		 Jackson Immuno
Research Laboratories, INC.		 Cat#703-545-155,
RRID:AB_2340375 		IF(1:500)
Antibody Alexa Fluor 647 AffiniPure anti-Rat IgG (H+L)
(Goat polyclonal)		 Jackson Immuno Research Laboratories, INC. Cat#112-605-167
RRID:AB_2338404 		IF(1:500)
Antibody anti-Mouse IgG (H+L) Highly Cross-Adsorbed
Secondary Antibody, Alexa Fluor 488
(Goat polyclonal)		 ThermoFisher
Scientific		 Cat#A-11029,
RRID:AB_2534088 		IF(1:500)
Antibody anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Goat polyclonal) ThermoFisher Scientific Cat#A-11034,
RRID:AB_2576217 		IF(1:500)
Antibody anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 546 (Goat polyclonal) ThermoFisher Scientific Cat#A-11035,
RRID:AB_2534093 		IF(1:500)
Other Rhodamine Phalloidin ThermoFisher Scientific Cat#R415 IF(1:100)
Genetic reagent (D. melanogaster) brat11/CyO, Actin-GFP Lee et al., 2006b doi: 10.1016/j.devcel.2006.01.017.
Genetic reagent (D. melanogaster) w1118; Df(2L)Exel8040/CyO Bloomington
Drosophila Stock Center		 BDSC: 7847
FlyBase: FBst0007847;
RRID:BDSC_7847 		FlyBase symbol: Df(2L)Exel8040/CyO
Genetic reagent (D. melanogaster) y1, M{vas-int.Dm}ZH-2A w*; M{UAS-insbFL-myc}ZH-86Fb Komori et al., 2018 doi: 10.1101/gad.320333.118.
Genetic reagent (D. melanogaster) Wor-Gal4(II) Lee et al., 2006a doi: 10.1038/nature04299.
Genetic reagent (D. melanogaster) Wor-Gal4(III) Weng et al., 2010 doi: 10.1016/j.devcel.2009.12.007.
Genetic reagent (D. melanogaster) y1, w*; P{tubPGAL80} LL10, P{neoFRT}40A/CyO Bloomington Drosophila Stock Center BDSC: 5192
FlyBase: FBst0005192;
RRID:BDSC_5192 		FlyBase symbol: y1, w*; P{tubPGAL80} LL10, P{neoFRT}40A/CyO
Genetic reagent (D. melanogaster) P{hsFLP}1, P{tubP-GAL80}LL1, w*,
P{neoFRT}19A; P{UAS-mCD8::GFP.L}LL5 		Bloomington Drosophila Stock Center BDSC: 5134
FlyBase: FBst0005134;
RRID:BDSC_5134_ 		FlyBase symbol: P{hsFLP}1, P{tubP-GAL80}LL1, w*,
P{neoFRT}19A; P{UAS-mCD8::GFP.L}LL5
Genetic reagent (D. melanogaster) y1 w*; PBac{y[+mDint2] w[+mC]=tll EGFP.S}VK00037 Bloomington Drosophila Stock Center BDSC: 30874
FlyBase: FBst0030874;
RRID:BDSC_30874 		FlyBase symbol: y1 w*; PBac{y[+mDint2] w[+mC]=tll EGFP.S}VK00037
Genetic reagent (D. melanogaster) Erm-Gal4 (II) Pfeiffer et al., 2008 doi: 10.1073/pnas.0803697105.
Genetic reagent (D. melanogaster) Erm-Gal4 (III) Pfeiffer et al., 2008 doi: 10.1073/pnas.0803697105.
Genetic reagent (D. melanogaster) tllRNAi: y1 sc* v1 sev21; P{TRiP.HMS01316}attP2 Bloomington Drosophila Stock Center BDSC: 34329
FlyBase: FBst0034329;
RRID:BDSC_34329 		FlyBase symbol: y1 sc* v1 sev21; P{TRiP.HMS01316}attP2
Genetic reagent (D. melanogaster) M{UAS-tll.ORF-VN}ZH-86Fb FlyORF F004752 FBst0502964;
RRID:FlyORF_ F004752		 FlyBase symbol: M{UAS-tll.ORF-VN}ZH-86Fb
Genetic reagent (D. melanogaster) Ase-Gal80 (II) Neumüller et al., 2011 doi: 10.1016/j.stem.2011.02.022.
Genetic reagent (D. melanogaster) erm1/CyO, Act-GFP Weng et al., 2010 doi: 10.1016/j.devcel.2009.12.007.
Genetic reagent (D. melanogaster) erm2/CyO, Act-GFP Weng et al., 2010 doi: 10.1016/j.devcel.2009.12.007.
Genetic reagent (D. melanogaster) UAS-erm Weng et al., 2010 doi: 10.1016/j.devcel.2009.12.007.
Genetic reagent (D. melanogaster) PBac{erm-flag4C(g)}VK33 Janssens and Lee, 2014 doi: 10.1242/dev.106534.
Genetic reagent (D. melanogaster) DRNAi: y1 v1; P{TRiP.JF02115}attP2 Bloomington Drosophila Stock Center BDSC: 26217
FlyBase: FBst0026217;
RRID:BDSC_26217 		FlyBase symbol: y1 v1; P{TRiP.JF02115}attP2
Genetic reagent (D. melanogaster) AseRNAi: y1 sc* v1 sev21; P{TRiP.HMS02847}attP2 Bloomington Drosophila Stock Center BDSC: 44552
FlyBase: FBst0044552;
RRID:BDSC_44552 		FlyBase symbol: y1 sc* v1 sev21; P{TRiP.HMS02847}attP2
Genetic reagent (D. melanogaster) hamRNAi: y1 v1; P{TRiP.JF02270}attP2 Bloomington Drosophila Stock Center BDSC: 26728
FlyBase: FBst0026728;
RRID:BDSC_26728 		FlyBase symbol: y1 v1; P{TRiP.JF02270}attP2
Genetic reagent (D. melanogaster) hamRNAi: y1 sc* v1 sev21; P{y[+t7.7] v[+t1.8]=TRiP.HMS00470}attP2 Bloomington Drosophila Stock Center BDSC: 32470
FlyBase: FBst0032470;
RRID:BDSC_32470 		FlyBase symbol: y1 sc* v1 sev21; P{y[+t7.7] v[+t1.8]=TRiP.HMS00470}attP2
Genetic reagent (D. melanogaster) OpaRNAi: y1 sc* v1 sev21; P{TRiP.HMS01185}attP2/TM3, Sb1 Bloomington Drosophila Stock Center BDSC: 34706
FlyBase: FBst0034706;
RRID:BDSC_34706 		FlyBase symbol: y1 sc* v1 sev21; P{TRiP.HMS01185}attP2/TM3, Sb1
Genetic reagent (D. melanogaster) w1118; Df(2L)Exel7071/CyO Bloomington Drosophila Stock Center BDSC: 7843
FlyBase: FBst0007843;
RRID:BDSC_7843 		FlyBase symbol: w1118; Df(2L)Exel7071/CyO
Genetic reagent (D. melanogaster) hamSKI, FRT40A/CyO This paper A new hamlet mutant fly line
Genetic reagent (D. melanogaster) P{w[+mW.hs]=GawB}elav[C155],
P{w[+mC]=UAS-mCD8::GFP.L}Ptp4E[LL4],
P{ry[+t7.2]=hsFLP}1, w[*] 		Bloomington Drosophila Stock Center BDSC: 5146
FlyBase: FBst0005146;
RRID:BDSC_5146 		FlyBase symbol: P{w[+mW.hs]=GawB}elav[C155],
P{w[+mC]=UAS-mCD8::GFP.L}Ptp4E[LL4],
P{ry[+t7.2]=hsFLP}1, w[*]
Genetic reagent (D. melanogaster) w1118; P{w[+mC]=UAS-Dcr-2.D}2 Bloomington Drosophila Stock Center BDSC: 24650
FlyBase: FBst00024650;
RRID:BDSC_24650 		FlyBase symbol: w1118; P{w[+mC]=UAS-Dcr-2.D}2
Genetic reagent (D. melanogaster) w*; P{w[+mC]=tubP- GAL8ts}2/TM2 Bloomington Drosophila Stock Center BDSC: 7017
FlyBase: FBst00024650;
RRID:BDSC_7017 		FlyBase symbol: w*; P{w[+mC]=tubP- GAL8ts}2/TM2
Genetic reagent (D. melanogaster) ham1,FRT40A/Cyo Moore et al., 2002 doi: 10.1126/science.1072387.
Genetic reagent (D. melanogaster) UAS-ham Moore et al., 2002 doi: 10.1126/science.1072387.
Genetic reagent (D. melanogaster) RNAi of Notch: y1, v1; P{y+t7.7v+t1.8=TRiP.HMS00001}attP2 Bloomington Drosophila Stock Center BDSC: 33611
FlyBase: FBst0033611;
RRID:BDSC_33611 		FlyBase symbol: y1, v1; P{y+t7.7v+t1.8=TRiP.HMS00001}attP2
Genetic reagent (D. melanogaster) Oregon-R-C Bloomington Drosophila Stock Center BDSC: 5
FlyBase: FBst0000005;
RRID:BDSC_5 		FlyBase symbol: Oregon-R-C
Genetic reagent (D. melanogaster) P{hsFLP}1, y1 w*; P{UAS- N.intra.GS}2/CyO; MKRS/TM2 Bloomington Drosophila Stock Center BDSC: 52008
FlyBase: FBst0052008;
RRID:BDSC_52008 		FlyBase symbol: P{hsFLP}1, y1 w*; P{UAS- N.intra.GS}2/CyO; MKRS/TM2
Genetic reagent (D. melanogaster) y1; M{vas-int.Dm}ZH-2A w*;
M{UAS-ham∆C-ZF-myc}ZH-86Fb 		This paper Transgene expressing Hamlet mutant form of the C-terminal zinc finger deletion version
Genetic reagent (D. melanogaster) y1; M{vas-int.Dm}ZH-2A w*; M{UAS-ERD::hamN-ZF-myc}ZH-86Fb This paper Transgene expressing Hamlet the N-terminal zinc finger fused with ERD transcriptional repression domain
Genetic reagent (D. melanogaster) y1; M{vas-int.Dm}ZH-2A w*;
M{UAS-VP-16::hamN-ZF-myc}ZH-86Fb 		This paper Transgene expressing Hamlet the N-terminal zinc finger fused with VP16 transcriptional activatoin domain
Genetic reagent (D. melanogaster) cu1, tll49/TM3, P{ftz/lacC}SC1, Sb1, Ser1 Bloomington Drosophila Stock Center BDSC: 7093
FlyBase: FBst007093;
RRID:BDSC_7093 		FlyBase symbol: cu1, tll49/TM3, P{ftz/lacC}SC1, Sb1, Ser1
Genetic reagent (D. melanogaster) st1 e1 tll1/TM3, Sb1 Bloomington Drosophila Stock Center BDSC: 2729
FlyBase: FBst002729;
RRID:BDSC_2729 		FlyBase symbol: st1 e1 tll1/TM3, Sb1
Genetic reagent (D. melanogaster) hamSK4, FRT40A/CyO Bloomington Drosophila Stock Center BDSC: 34329FlyBase: FBst0034329;RRID:BDSC_34329 FlyBase symbol: y1 sc* v1 sev21; P{TRiP.HMS01316}attP2
Genetic reagent (D. melanogaster) hdac3RNAi: y1 sc* v1 sev21; P{TRiP.HMS00087}attP2 Bloomington Drosophila Stock Center BDSC: 34778
FlyBase: FBst0034778;
RRID:BDSC_34778 		FlyBase symbol: hdac3RNAi: y1 sc* v1sev21; P{TRiP.HMS00087}attP2
Genetic reagent (D. melanogaster) Su(z)12RNAi: y1 sc* v1 sev21; P{TRiP.HMS00280}attP2/TM3, Sb1 Bloomington Drosophila Stock Center BDSC: 33402
FlyBase: FBst0033402;
RRID:BDSC_33402 		FlyBase symbol: y1 sc* v1 sev21; P{TRiP.HMS00280}attP2/TM3, Sb1
Genetic reagent (D. melanogaster) Su(var)3-3RNAi: y1 sc* v1 sev21; P{TRiP.HMS00638}attP2 Bloomington Drosophila Stock Center BDSC: 32853
FlyBase: FBst0032853;
RRID:BDSC_32853 		FlyBase symbol: y1 sc* v1 sev21; P{TRiP.HMS00638}attP2
Genetic reagent (D. melanogaster) Su(var)205RNAi: y1 sc* v1 sev21; P{TRiP.GL00531}attP40 Bloomington Drosophila Stock Center BDSC: 36792
FlyBase: FBti0146447;
RRID:BDSC_36792 		FlyBase symbol: y1 sc* v1 sev21; P{TRiP.GL00531}attP40
Genetic reagent (D. melanogaster) UAS-Mi-2DN Kovač et al., 2018 doi: 10.1038/s41467-018-04503-2.
Genetic reagent (D. melanogaster) UAS-Mi-brmDN Herr et al., 2010 doi: 10.1016/j.ydbio.2010.04.006.
Genetic reagent (D. melanogaster) In(1)wm4; Su(var)3–91/TM3, Sb1 Ser1 Bloomington Drosophila Stock Center BDSC: 6209
FlyBase: FBst0006209;
RRID:BDSC_6209 		FlyBase symbol: In(1)wm4; Su(var)3–91/TM3, Sb1 Ser1
Genetic reagent (D. melanogaster) w1118; PBac{Sp1- EGFP.S}VK00033 Bloomington Drosophila Stock Center BDSC: 38669
FlyBase: FBst0038669;
RRID:BDSC_38669 		FlyBase symbol: w1118; PBac{Sp1- EGFP.S}VK00033
Sequenced-based reagent Ham_F Eroglu et al., 2014 doi: 10.1016/j.cell.2014.01.053. PCR primers atagatcctttggccagcagac
Sequenced-based reagent Ham_R Eroglu et al., 2014 doi: 10.1016/j.cell.2014.01.053. PCR primers agtactcctccctttcggcaat
Sequenced-based reagent Ase_F Komori et al., 2014b doi: 10.7554/eLife.03502. PCR primers agcccgtgagcttctacgac
Sequenced-based reagent Ase_R Komori et al., 2014b doi: 10.7554/eLife.03502. PCR primers gcatcgatcatgctctcgtc
Sequenced-based reagent D_F This paper PCR primers gcggcggcggtcaacaat
Sequenced-based reagent D_R This paper PCR primers tgcggcgtacagcgaagggt
Sequenced-based reagent Erm_F Eroglu et al., 2014 doi: 10.1016/j.cell.2014.01.053. PCR primers gttacggccaggcatcgggtcaa
Sequenced-based reagent Erm_R Eroglu et al., 2014 doi: 10.1016/j.cell.2014.01.053. PCR primers gggccaggcgggattactcgtctc
Sequenced-based reagent PntP1_F Komori et al., 2014b doi: 10.7554/eLife.03502. PCR primers ggcagtacgggcagcaccac
Sequenced-based reagent PntP1_R Komori et al., 2014b doi: 10.7554/eLife.03502. PCR primers ctcaacgcccccaccagatt
Sequenced-based reagent Dpn_F Komori et al., 2014b doi: 10.7554/eLife.03502.Komori et al., 2014b PCR primers catcatgccgaacacaggtt
Sequenced-based reagent Dpn_R Komori et al., 2014b PCR primers gaagattggccggaactgag
Recombinant DNA reagent pUAST-ham∆C-ZF-myc-attB (plasmid) This paper Plasmid DNA of a transgene expressing Hamlet mutant form of the C-terminal zinc finger deletion version
Recombinant DNA reagent pUAST-ERD::hamN-ZF-myc-attB (plasmid) This paper Plasmid DNA of a transgene expressing Hamlet the N-terminal zinc finger fused with ERD transcriptional repression domain
Recombinant DNA reagent pUAST-VP16::hamN-ZF-myc-attB (plasmid) This paper Plasmid DNA of a transgene expressing Hamlet the N-terminal zinc finger fused with VP16 transcriptional activatoin domain
Software, algorithm LAS AF Leica Microsystems RRID:SCR_013673
Software, algorithm ImageJ 1.50 g National Institute of Health RRID:SCR_003070