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. 2020 Dec 2;9:e61487. doi: 10.7554/eLife.61487

Figure 4. Heatmap of DNA-binding of p53 DBD mutants.

Affinity of mutant DBD for DNA oligonucleotides, expressed relative to WT DBD (4°C). Oligonucleotide names and Hill coefficients (n) are on the left. DNA sequences are in Supplementary file 1A.

Figure 4—source data 1. KDNA values used to generate the heatmap in Figure 4.
KDNA values are reported as mean ± SE in units of μM. Values are derived Hill fitting of fluorescence anisotropy binding curves. Each oligonucleotide was separately analyzed by global fitting across all mutants with floating KDNA and a linked Hill parameter, the value of which is indicated by the bracketed numbers in the top row. Replicates (indicated in the first column) consisted of independent experiments performed with the same preparations of purified proteins and oligonucleotides. Each replicate was fitted with its own KDNA, and the results were pooled.

Figure 4.

Figure 4—figure supplement 1. DNA-binding of p53 DBD mutants.

Figure 4—figure supplement 1.

(A) Representative fits for DBD mutants binding Cy3-labeled p53RE oligonucleotides. (B) Representative curves illustrating restoration of DNA-binding (waf1) for DBD mutants by EGTA-buffered [Zn2+]free.