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. 2020 Dec 2;9:e61487. doi: 10.7554/eLife.61487

Key resources table.

Reagent type
(species)
or resource
Designation Source or
reference
Identifiers Additional
information
Antibody Anti-p53 PAB240 (mouse monoclonal) EMD Chemicals OP29 1:400 for IF, 2 μg for IP
Antibody Anti-p53 PAB1620 (mouse monoclonal) EMD Chemicals OP33 1:50 for IF, 2 μg for IP
Antibody Goat anti-mouse (goat polyclonal, HRP conjugate) Santa Cruz Biotechnology sc-2005 1:3000 for western blot
Antibody Anti-p53 (DO-1) (mouse monoclonal) Santa Cruz Biotechnology sc-126 1:1000 for western blot
Antibody Anti-p53 (DO-7) (mouse monoclonal) Santa Cruz Biotechnology sc-47698 1:1000 for western blot
Antibody Anti-beta-actin (AC-15) (mouse monoclonal) Santa Cruz Biotechnology sc-69879 1:2000 for western blot
Commercial assay or kit Q5 Site-Directed Mutagenesis Kit NEB E0554S Site-directed mutagenesis
Sequence-based reagent TaqMan assay human p21 ThermoFisher Hs00355782_m1
Sequence-based reagent TaqMan assay human PUMA ThermoFisher Hs00248075_m1
Sequence-based reagent TaqMan assay human NOXA ThermoFisher Hs00560402_m1
Sequence-based reagent TaqMan assay human beta-actin ThermoFisher Hs99999903_m1
Chemical compound, drug ZMC1 Synthesized in this work
Genetic reagent (M. musculus) Female CR ATH HO (order when 6–8 weeks old) Charles River Laboratories