Key resources table.
Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
---|---|---|---|---|
Antibody | Anti-p53 PAB240 (mouse monoclonal) | EMD Chemicals | OP29 | 1:400 for IF, 2 μg for IP |
Antibody | Anti-p53 PAB1620 (mouse monoclonal) | EMD Chemicals | OP33 | 1:50 for IF, 2 μg for IP |
Antibody | Goat anti-mouse (goat polyclonal, HRP conjugate) | Santa Cruz Biotechnology | sc-2005 | 1:3000 for western blot |
Antibody | Anti-p53 (DO-1) (mouse monoclonal) | Santa Cruz Biotechnology | sc-126 | 1:1000 for western blot |
Antibody | Anti-p53 (DO-7) (mouse monoclonal) | Santa Cruz Biotechnology | sc-47698 | 1:1000 for western blot |
Antibody | Anti-beta-actin (AC-15) (mouse monoclonal) | Santa Cruz Biotechnology | sc-69879 | 1:2000 for western blot |
Commercial assay or kit | Q5 Site-Directed Mutagenesis Kit | NEB | E0554S | Site-directed mutagenesis |
Sequence-based reagent | TaqMan assay human p21 | ThermoFisher | Hs00355782_m1 | |
Sequence-based reagent | TaqMan assay human PUMA | ThermoFisher | Hs00248075_m1 | |
Sequence-based reagent | TaqMan assay human NOXA | ThermoFisher | Hs00560402_m1 | |
Sequence-based reagent | TaqMan assay human beta-actin | ThermoFisher | Hs99999903_m1 | |
Chemical compound, drug | ZMC1 | Synthesized in this work | ||
Genetic reagent (M. musculus) | Female CR ATH HO (order when 6–8 weeks old) | Charles River Laboratories |