Skip to main content
. Author manuscript; available in PMC: 2020 Dec 10.
Published in final edited form as: Pharm Res. 2014 Oct 8;32(3):1141–1157. doi: 10.1007/s11095-014-1525-x

Table II.

Sequences and MRM transitions of signature peptides for quantifying CYP and UGT isofroms

Isoforms Peptides Sequence Mass Quantitative Ions Qualitative Ions
MRM Transition Ion Seriesa DP CE CXP MRM Transition Ion Series DP CE CXP
CYP1A2 Y244LPNPALQR252 1071.3 536.4 > 398.2 y3° 90 20 20 536.4 > 795.9 y7 90 25 20
536.4 > 584.6 y5 90 25 15 536.4 > 277.3 b2 90 20 15
CYP2A6 G162TGGANIDPTFFLSR176 1552.7 777.0 > 867.1 y7 50 40 40 777.0 > 441.2 b10* 50 35 20
777.0 > 982.1 y8 50 35 45 777.0 > 554.1 b7* 50 30 25
CYP2B6 G110YGVIFANGNR120 1167.3 585.0 > 792.5 y7 50 20 30 585.0 > 679.2 y6 50 20 30
585.0 > 278.2 b3 50 30 15 585.0 > 377.5 b4 50 20 15
CYP2C8 G98NSPISQR105 875.9 429.5 > 390.4 y3 50 25 20 429.5 > 302.9 y2 50 23 25
429.5 > 503.4 y4 50 25 20 429.5 > 599.8 y5 50 20 40
CYP2C9 G98IFPLAER105 902.7 451.8 > 585.4 y5 90 18 30 451.8 > 318.1 b3 90 15 15
451.8 > 366.8 y6++ 90 15 15 451.8 > 488.3 y4 90 25 20
CYP2C19 G98HFPLAER105 926.92 463.8 > 195.2 b2 50 25 12 463.8 > 342.2 b3 50 25 20
463.8 > 585.2 y5 50 25 30 463.8 > 732.3 y6 50 22 35
CYP2D6 D381IEVQGFR388 963.1 482.3 > 735.3 y6 50 20 35 482.3 > 378.9 y3 50 20 25
482.3 > 507.3 y4 50 17 30 482.3 > 606.3 y5 50 17 35
CYP2E1 G113IIFNNGPTWK123 1246.4 623.9 > 538.7 y9++ 50 20 35 623.9 > 284.1 b3 50 20 15
623.9 > 963.1 y8 50 25 45 623.9 > 816.7 y7 50 25 35
CYP3A4/3A43 L331QEEIDAVLPNK342 1368.6 684.7 > 242.0 b2 50 30 20 684.7 > 358.6 y3 50 25 15
684.7 > 1127.6 y10 50 25 45 684.7 > 471.2 y4 50 30 25
CYP3A43 F337ALTNIK343 806.0 404.0 > 191.0 a2 90 20 12 404.0 > 588.3 y5 90 19 27
404.0 > 219.0 b2 90 18 14 404.0 > 659.3 y6 90 20 32
UGT1A1 T78YPVPFQR85 1007.2 504.1 > 372.3 y6++ 50 16 15 504.1 > 547.4 y4 50 26 25
504.1 > 237.0 a2 50 20 20 504.1 > 743.7 y6 50 18 15
UGT1A4 V92TLGYTQGFFETEHLLK108 1983.3 662.0 > 201.1 b2 90 25 13 662.0 > 296.3 b3° 90 25 13
662.0 > 892.2 y15++ 90 25 38 662.0 > 835.6 y14++ 90 25 37
UGT1A6 D44IVEVLSDR52 1045.2 523.5 > 490.1 y4 50 20 27 523.5 > 718.5 y6 50 20 26
523.5 > 229.1 b2 50 25 12 523.5 > 817.4 y7 50 23 35
UGT1A9 A92FAHAQWK99 958.1 320.4 > 191.0 a2 90 15 13 320.4 > 335.2 y5++ 90 18 15
320.4 > 370.8 y6++ 90 13 16 320.4 > 444.3 y3* 90 14 18
UGT2B7 T41ILDELIQR49 1100.3 550.7 > 187.4 a2 90 27 10 550.7 > 773.6 y6 90 20 25
550.7 > 215.1 b2 90 20 15 550.7 > 886.0 y7 90 22 14
ISb GYLPNPALQR 1128.3 565.0 > 221.1 b2 90 26 14 565.0 > 398.2 y3° 90 22 20
565.0 > 454.9 y8++ 90 22 18 565.0 > 584.2 y5 90 31 27
a

The nomenclature, reported by Roepstorff [32] and Johnson [33], for fragment ions in mass spectrum of signature peptides was used.

b

Only one MRM transition (565.0 > 221.1) was used as quantitative ion for the quantification of internal standard (IS).