Table III. Precision and accuracy for the determination of CYP and UGT isoforms.
Intra- and inter-day precision and accuracy were determined by measuring standard samples at three concentration levels (Low, Mid, High): 2.3, 18.8 and 150 nM for CYP2C9; 1.6, 12.5 and 100nM for CYP2E1; and 0.9, 7.5 and 60 nM for other signature peptides, respectively.
| Isoforms | LLOQ [nM] | Accuracy | Intra-day Precision | Inter-day Precision | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| [%Deviation] | [%RSD] | [%RSD] | ||||||||||
| Low | Mid | High | Low | Mid | High | Low | Mid | High | ||||
| CYP1A2 | 0.1 | 10.4 | 10.9 | 5.2 | 2.6 | 4.4 | 6.5 | 4.8 | 4.0 | 4.1 | ||
| CYP2A6 | 0.1 | 8.3 | 14.5 | 14.2 | 7.1 | 4.6 | 6.2 | 6.8 | 5.5 | 5.1 | ||
| CYP2B6 | 0.2 | 18.2 | 16.0 | 12.0 | 7.5 | 11.3 | 11.3 | 9.2 | 7.2 | 7.3 | ||
| CYP2C8 | 0.4 | 16.3 | 12.9 | 6.1 | 5.1 | 10.4 | 6.6 | 9.9 | 10.7 | 4.9 | ||
| CYP2C9 | 0.1 | 17.0 | 18.0 | 5.0 | 3.5 | 6.3 | 6.2 | 5.7 | 4.5 | 3.9 | ||
| CYP2C19 | 0.6 | 27.9 | 25.6 | 22.9 | 13.4 | 7.6 | 5.7 | 10.3 | 5.8 | 5.0 | ||
| CYP2D6 | 0.1 | 19.4 | 12.4 | 7.4 | 3.1 | 7.1 | 7.1 | 5.4 | 6.5 | 4.9 | ||
| CYP2E1 | 0.3 | −0.5 | 9.8 | 18.4 | 9.7 | 19.2 | 6.0 | 12.8 | 21.5 | 10.3 | ||
| CYP3A4/3A43 | 0.2 | 12.7 | 18.0 | 16.2 | 3.9 | 4.0 | 6.2 | 8.8 | 7.1 | 5.8 | ||
| CYP3A43 | 0.1 | 5.0 | 13.0 | 8.7 | 4.0 | 4.6 | 6.5 | 6.0 | 4.5 | 5.0 | ||
| UGT1A1 | 0.1 | 28.4 | 11.3 | 7.6 | 4.0 | 5.1 | 5.9 | 5.6 | 4.6 | 4.6 | ||
| UGT1A4 | 0.1 | −9.6 | 13.0 | 29.5 | 8.7 | 5.6 | 8.1 | 16.4 | 10.0 | 10.2 | ||
| UGT1A6 | 0.4 | 18.4 | 7.2 | 15.1 | 8.7 | 6.4 | 6.8 | 10.3 | 10.9 | 8.0 | ||
| UGT1A9 | 0.1 | 5.3 | 14.3 | 12.6 | 6.9 | 6.5 | 6.1 | 11.2 | 10.9 | 9.6 | ||
| UGT2B7 | 0.1 | 12.6 | 14.0 | 7.6 | 4.5 | 5.7 | 7.4 | 6.5 | 9.5 | 9.7 | ||