Table V. Validation for LC-MS/MS quantification of protein amount of CYPs and UGTs using standard addition method.
Isoforms | Amount in nHLMs (mean ± SD, n=8) [pmol / sample] | Spiked Amount of Peptide [pmol / sample] | Calculated Amount of Spiked Peptide [pmol / sample] | Inaccuracy [%Deviation] |
---|---|---|---|---|
CYP1A2 | 2.5±0.5 | 2.9 | 2.9±0.2 | −11% ~ 8% |
CYP2A6 | 2.5±1.4 | 3.9 | 3.6±0.3 | −22% ~ 2% |
CYP2B6 | 0.1±0.05 | 0.8 | 0.7±0.05 | −15% ~ 4% |
CYP2C8 | 6.7±3.8 | 9.4 | 9.1±0.6 | −15% ~ 4% |
CYP2C9 | 10.1±5.1 | 13.4 | 11.6±1.0 | −24% ~ −3% |
CYP2C19 | 0.5±1.0 | 2.2 | 2.4±0.1 | −1% ~ 16% |
CYP2D6 | 1.5±0.9 | 2.3 | 2.0±0.2 | −27% ~ −4% |
CYP2E1 | 8.4±2.1 | 22.1 | 21±1.6 | −17% ~ 4% |
CYP3A4/3A43 | 7.0±5.0 | 11.4 | 11.5±0.9 | −9% ~ 11% |
CYP3A43 | <0.01±0.0 | 1.0 | 1.0±0.1 | −1% ~ 13% |
UGT1A1 | 1.4±0.5 | 3.4 | 3.3±0.2 | −16% ~ 1% |
UGT1A4 | 4.3±1.8 | 14.6 | 14.1±1.0 | −13% ~ 7% |
UGT1A6 | 1.2±0.7 | 3.7 | 3.4±0.1 | −18% ~ −4% |
UGT1A9 | 2.0±1.1 | 7.5 | 7.1±0.5 | −14% ~ 3% |
UGT2B7 | 2.8±1.5 | 5.4 | 5.8±0.4 | −7% ~ 16% |