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The threespine stickleback CEN repeat hybridizes to the centromere. FISH reveals that the CEN probe (green) hybridizes to: (a) distinct puncta in interphase nuclei from Lake Union embryos; (b) a single region on each chromosome except one (arrow) in a metaphase spread from a Lake Union male; and (c) the primary constriction (arrowheads) on each chromosome in a higher magnification view of the boxed region in panel (b). Scale bar, 5 μm
