Skip to main content
. 2020 Jul 1;27:155–164. doi: 10.1016/j.jare.2020.06.023

Fig. 1.

Fig. 1

H2S blocked FOXO1 inactivation in ET-1-stimulated VSMCs. a. The phosphorylation of FOXO1 in ET-1-stimulated VSMCs incubated with H2S at different concentrations (50–200 μM) for 24 h. b. The phosphorylation of FOXO1 in ET-1-stimulated VSMCs incubated with 100 μM of H2S for different time (6–24 h). c. The phosphorylation of FOXO1 in VSMCs detected by Western blotting. d. FOXO1 nuclear exclusion to the cytoplasm in VSMCs determined by confocal immunofluorescence. Blue color indicates nuclei, and green color indicates FOXO1-positive signal. e. The PCNA mRNA level in VSMCs detected by qRT-PCR. f. PCNA expression in VSMCs detected by Western blotting. g. PCNA expression in VSMCs detected by confocal immunofluorescence. The blue color indicates nuclei, and the red color indicates PCNA-positive signals. h. VSMC proliferation detected by CCK-8 assay. i. VSMC proliferation evaluated by counting cell number. At least three times of replicates of experiments were conducted. ** p < 0.01, * p < 0.05 vs Con group; ## p < 0.01, # p < 0.05 vs ET-1 group. The data were shown as means ± SEMs.